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guinea pig polyclonal serum anti calbindin d28k  (Synaptic Systems)


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    Synaptic Systems guinea pig polyclonal serum anti calbindin d28k
    ( A ) Two-photon laser scanning microscopy (2PLSM) image of the outermost cerebellar molecular layer in vivo shows cytoplasmic GCaMP3 and parasagittally aligned MLI neurites in a horizontal plane of focus. ( B ) Confocal stack projections of sagittal cerebellar slices from mice expressing AAV2/1hSyn.Flex.GCaMP3 on the background of PV-promoter driven CRE recombinase. Left : slice stained with an antibody against <t>calbindin,</t> a protein expressed in PCs and not in MLIs. Note that calbindin positive PCs do not express GCaMP3. Right : slice stained with an antibody against PV, a protein expressed in both PCs and MLIs. Note the colocalization between GCaMP3 and PV positive MLIs. ( C ) A beam pattern of Ca i rise is illustrated by the ΔF/Fo image at the peak of the fluorescence increase evoked in the molecular layer by a 0.5 s long PF stimulation at 100 Hz (horizontal plane of focus) using a bi-polar theta glass pipette as depicted in the cartoon. The highest value for the pseudo-color scale is 100%. ( D ) Time course of the somatic MLI Ca i signals evoked in a representative MLI by 100 Hz PF stimulations of various durations. The arrow indicates the time of stimulation onset. The inset shows an average of pre-stimulus images to illustrate the ROI analyzed. ( E ) Average peak ΔF/Fo values from 18 somata, normalized to the peak value for 200 ms trains at 100 Hz.
    Guinea Pig Polyclonal Serum Anti Calbindin D28k, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guinea+pig+polyclonal+serum+anti+calbindin+d28k/guinea+pig+anti+calbindin/pmc07220378-302-43-45
    Average 90 stars, based on 1 article reviews
    guinea pig polyclonal serum anti calbindin d28k - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Synergism of type 1 metabotropic and ionotropic glutamate receptors in cerebellar molecular layer interneurons in vivo"

    Article Title: Synergism of type 1 metabotropic and ionotropic glutamate receptors in cerebellar molecular layer interneurons in vivo

    Journal: eLife

    doi: 10.7554/eLife.56839

    ( A ) Two-photon laser scanning microscopy (2PLSM) image of the outermost cerebellar molecular layer in vivo shows cytoplasmic GCaMP3 and parasagittally aligned MLI neurites in a horizontal plane of focus. ( B ) Confocal stack projections of sagittal cerebellar slices from mice expressing AAV2/1hSyn.Flex.GCaMP3 on the background of PV-promoter driven CRE recombinase. Left : slice stained with an antibody against calbindin, a protein expressed in PCs and not in MLIs. Note that calbindin positive PCs do not express GCaMP3. Right : slice stained with an antibody against PV, a protein expressed in both PCs and MLIs. Note the colocalization between GCaMP3 and PV positive MLIs. ( C ) A beam pattern of Ca i rise is illustrated by the ΔF/Fo image at the peak of the fluorescence increase evoked in the molecular layer by a 0.5 s long PF stimulation at 100 Hz (horizontal plane of focus) using a bi-polar theta glass pipette as depicted in the cartoon. The highest value for the pseudo-color scale is 100%. ( D ) Time course of the somatic MLI Ca i signals evoked in a representative MLI by 100 Hz PF stimulations of various durations. The arrow indicates the time of stimulation onset. The inset shows an average of pre-stimulus images to illustrate the ROI analyzed. ( E ) Average peak ΔF/Fo values from 18 somata, normalized to the peak value for 200 ms trains at 100 Hz.
    Figure Legend Snippet: ( A ) Two-photon laser scanning microscopy (2PLSM) image of the outermost cerebellar molecular layer in vivo shows cytoplasmic GCaMP3 and parasagittally aligned MLI neurites in a horizontal plane of focus. ( B ) Confocal stack projections of sagittal cerebellar slices from mice expressing AAV2/1hSyn.Flex.GCaMP3 on the background of PV-promoter driven CRE recombinase. Left : slice stained with an antibody against calbindin, a protein expressed in PCs and not in MLIs. Note that calbindin positive PCs do not express GCaMP3. Right : slice stained with an antibody against PV, a protein expressed in both PCs and MLIs. Note the colocalization between GCaMP3 and PV positive MLIs. ( C ) A beam pattern of Ca i rise is illustrated by the ΔF/Fo image at the peak of the fluorescence increase evoked in the molecular layer by a 0.5 s long PF stimulation at 100 Hz (horizontal plane of focus) using a bi-polar theta glass pipette as depicted in the cartoon. The highest value for the pseudo-color scale is 100%. ( D ) Time course of the somatic MLI Ca i signals evoked in a representative MLI by 100 Hz PF stimulations of various durations. The arrow indicates the time of stimulation onset. The inset shows an average of pre-stimulus images to illustrate the ROI analyzed. ( E ) Average peak ΔF/Fo values from 18 somata, normalized to the peak value for 200 ms trains at 100 Hz.

    Techniques Used: Laser-Scanning Microscopy, In Vivo, Expressing, Staining, Fluorescence, Transferring

    Related Articles

    Incubation:

    Article Title: An excitatory GABA loop operating in vivo
    Article Snippet: Fifty micrometer thick transverse slices were prepared using a vibrating slicer (Leica VT 1000S, Leica Biosystems, Nussloch, Germany). .. The slices were first incubated in PBS containing 0.3% Triton and 10% fetal bovine serum for 5 h at room temperature then at 4°C overnight with guinea pig polyclonal serum anti calbindin D28k (Synaptic Systems, Göttingen, Germany) diluted to 1:1000 in PBS containing 1 mg/ml of Bovine Serum Albumine (BSA). .. After three washes in PBS, the slices were incubated for 3 h with secondary goat antibodies anti-guinea pig IgG conjugated to Alexa fluor 647 (1/500, Molecular Probes, Eugene, OR, USA).

    Article Title: Synergism of type 1 metabotropic and ionotropic glutamate receptors in cerebellar molecular layer interneurons in vivo
    Article Snippet: 50 mm thick parasagittal slices were prepared from the vermis using a vibrating slicer (Leica VT 1000S, Leica Microsystems, Germany). .. The slices were first incubated in PBS containing 0.3% Triton and 10% fetal bovine serum for 5 hr at room temperature then at 4 ̊C overnight with a mixture of rabbit polyclonal serum anti-Parvalbumin (PV25, SWANT, Marly, Switzerland) and guinea pig polyclonal serum anti calbindin D28k (Synaptic Systems, Gôttingen, Germany) diluted to 1/1000 in PBS containing 1 mg/ml of Bovine Serum Albumine (BSA). .. After 3 washes in PBS, the slices were incubated for 3 hr with secondary goat antibodies anti-rabbit IgG and anti-guinea pig IgG conjugated to Alexa fluor 546 and Alexa fluor 647 respectively (1/500, Molecular Probes, Eugene, OR).

    Article Title: Synergism of type 1 metabotropic and ionotropic glutamate receptors in cerebellar molecular layer interneurons in vivo
    Article Snippet: 50 μm thick parasagittal slices were prepared from the vermis using a vibrating slicer (Leica VT 1000S, Leica Microsystems, Germany). .. The slices were first incubated in PBS containing 0.3% Triton and 10% fetal bovine serum for 5 hr at room temperature then at 4°C overnight with a mixture of rabbit polyclonal serum anti-Parvalbumin (PV25, SWANT, Marly, Switzerland) and guinea pig polyclonal serum anti calbindin D28k (Synaptic Systems, Gôttingen, Germany) diluted to 1/1000 in PBS containing 1 mg/ml of Bovine Serum Albumine (BSA). .. After 3 washes in PBS, the slices were incubated for 3 hr with secondary goat antibodies anti-rabbit IgG and anti-guinea pig IgG conjugated to Alexa fluor 546 and Alexa fluor 647 respectively (1/500, Molecular Probes, Eugene, OR).



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    guinea pig polyclonal serum anti calbindin d28k  (Synaptic Systems)
    90
    Synaptic Systems guinea pig polyclonal serum anti calbindin d28k
    ( A ) Two-photon laser scanning microscopy (2PLSM) image of the outermost cerebellar molecular layer in vivo shows cytoplasmic GCaMP3 and parasagittally aligned MLI neurites in a horizontal plane of focus. ( B ) Confocal stack projections of sagittal cerebellar slices from mice expressing AAV2/1hSyn.Flex.GCaMP3 on the background of PV-promoter driven CRE recombinase. Left : slice stained with an antibody against <t>calbindin,</t> a protein expressed in PCs and not in MLIs. Note that calbindin positive PCs do not express GCaMP3. Right : slice stained with an antibody against PV, a protein expressed in both PCs and MLIs. Note the colocalization between GCaMP3 and PV positive MLIs. ( C ) A beam pattern of Ca i rise is illustrated by the ΔF/Fo image at the peak of the fluorescence increase evoked in the molecular layer by a 0.5 s long PF stimulation at 100 Hz (horizontal plane of focus) using a bi-polar theta glass pipette as depicted in the cartoon. The highest value for the pseudo-color scale is 100%. ( D ) Time course of the somatic MLI Ca i signals evoked in a representative MLI by 100 Hz PF stimulations of various durations. The arrow indicates the time of stimulation onset. The inset shows an average of pre-stimulus images to illustrate the ROI analyzed. ( E ) Average peak ΔF/Fo values from 18 somata, normalized to the peak value for 200 ms trains at 100 Hz.
    Guinea Pig Polyclonal Serum Anti Calbindin D28k, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guinea+pig+polyclonal+serum+anti+calbindin+d28k/guinea+pig+anti+calbindin/pmc07220378-302-43-45
    Average 90 stars, based on 1 article reviews
    guinea pig polyclonal serum anti calbindin d28k - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Two-photon laser scanning microscopy (2PLSM) image of the outermost cerebellar molecular layer in vivo shows cytoplasmic GCaMP3 and parasagittally aligned MLI neurites in a horizontal plane of focus. ( B ) Confocal stack projections of sagittal cerebellar slices from mice expressing AAV2/1hSyn.Flex.GCaMP3 on the background of PV-promoter driven CRE recombinase. Left : slice stained with an antibody against calbindin, a protein expressed in PCs and not in MLIs. Note that calbindin positive PCs do not express GCaMP3. Right : slice stained with an antibody against PV, a protein expressed in both PCs and MLIs. Note the colocalization between GCaMP3 and PV positive MLIs. ( C ) A beam pattern of Ca i rise is illustrated by the ΔF/Fo image at the peak of the fluorescence increase evoked in the molecular layer by a 0.5 s long PF stimulation at 100 Hz (horizontal plane of focus) using a bi-polar theta glass pipette as depicted in the cartoon. The highest value for the pseudo-color scale is 100%. ( D ) Time course of the somatic MLI Ca i signals evoked in a representative MLI by 100 Hz PF stimulations of various durations. The arrow indicates the time of stimulation onset. The inset shows an average of pre-stimulus images to illustrate the ROI analyzed. ( E ) Average peak ΔF/Fo values from 18 somata, normalized to the peak value for 200 ms trains at 100 Hz.

    Journal: eLife

    Article Title: Synergism of type 1 metabotropic and ionotropic glutamate receptors in cerebellar molecular layer interneurons in vivo

    doi: 10.7554/eLife.56839

    Figure Lengend Snippet: ( A ) Two-photon laser scanning microscopy (2PLSM) image of the outermost cerebellar molecular layer in vivo shows cytoplasmic GCaMP3 and parasagittally aligned MLI neurites in a horizontal plane of focus. ( B ) Confocal stack projections of sagittal cerebellar slices from mice expressing AAV2/1hSyn.Flex.GCaMP3 on the background of PV-promoter driven CRE recombinase. Left : slice stained with an antibody against calbindin, a protein expressed in PCs and not in MLIs. Note that calbindin positive PCs do not express GCaMP3. Right : slice stained with an antibody against PV, a protein expressed in both PCs and MLIs. Note the colocalization between GCaMP3 and PV positive MLIs. ( C ) A beam pattern of Ca i rise is illustrated by the ΔF/Fo image at the peak of the fluorescence increase evoked in the molecular layer by a 0.5 s long PF stimulation at 100 Hz (horizontal plane of focus) using a bi-polar theta glass pipette as depicted in the cartoon. The highest value for the pseudo-color scale is 100%. ( D ) Time course of the somatic MLI Ca i signals evoked in a representative MLI by 100 Hz PF stimulations of various durations. The arrow indicates the time of stimulation onset. The inset shows an average of pre-stimulus images to illustrate the ROI analyzed. ( E ) Average peak ΔF/Fo values from 18 somata, normalized to the peak value for 200 ms trains at 100 Hz.

    Article Snippet: The slices were first incubated in PBS containing 0.3% Triton and 10% fetal bovine serum for 5 hr at room temperature then at 4°C overnight with a mixture of rabbit polyclonal serum anti-Parvalbumin (PV25, SWANT, Marly, Switzerland) and guinea pig polyclonal serum anti calbindin D28k (Synaptic Systems, Gôttingen, Germany) diluted to 1/1000 in PBS containing 1 mg/ml of Bovine Serum Albumine (BSA).

    Techniques: Laser-Scanning Microscopy, In Vivo, Expressing, Staining, Fluorescence, Transferring